Robust determination of differential abundance in shotgun proteomics using nonparametric statistics
نویسندگان
چکیده
منابع مشابه
Analyzing marginal cases in differential shotgun proteomics
SUMMARY We present an approach to statistically pinpoint differentially expressed proteins that have quantitation values near the quantitation threshold and are not identified in all replicates (marginal cases). Our method uses a Bayesian strategy to combine parametric statistics with an empirical distribution built from the reproducibility quality of the technical replicates. AVAILABILITY Th...
متن کاملDifferential protein profile in sexed bovine semen: shotgun proteomics investigation.
The preparation of sexed semen is based on the differential DNA content between the X and Y chromosome bearing sperm cells determined by fluorescence-activated cell sorting. In spite of its intrinsic limitations this represents the only effective method. However, the employment of sexed sperm for breeding food producing animals on a large scale requires additional knowledge in the protein reper...
متن کاملShotgun Proteomics in Neuroscience
Mass spectrometry-based proteomics is increasingly used to address basic and clinical questions in biomedical research through studies of differential protein expression, protein-protein interactions, and posttranslational modifications. The complex structural and functional organization of the human brain warrants the application of high-throughput, systematic approaches to understand the func...
متن کاملEffect of Laparoscopic Gastric Plication on the Blood Protein Profile of Obese Subjects Using Shotgun Proteomics
Introduction: Nowadays, bariatric surgery is considered to be the most effective technique in the treatment of morbid obesity. In the current study, the effect of Laparoscopic Gastric Plication (LGP), a new technique, on the serum protein profile of obese patients has been investigated following surgery. Materials and Methods: Serum of 16 obese subjects with mean body mass index (BMI) of 41.2±5...
متن کاملShotgun proteomics using the iTRAQ isobaric tags.
Shotgun proteomic methods involving isobaric tagging of peptides enable high-throughput proteomic analysis. iTRAQ reagents allow simultaneous identification and quantitation of proteins in four different samples using tandem mass spectrometry (MS). In this article, we provide a brief description of proteome analysis using iTRAQ reagents and review the current applications of these reagents in p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Omics
سال: 2018
ISSN: 2515-4184
DOI: 10.1039/c8mo00077h